Explanation HCV RNA assays (quantitative)
Quantitative methods of determining HCV researchamount of virus in 1 ml of blood and used to determine the treatment plan and detecting the selected therapy effectiveness. Quantitative HCV research include:
- Polymerase chain reaction (PCR) and RT-PCR;
- BDNA (branched DNA);
- TMA (transcription-mediated amplification).
RT-PCR refers to the polymerase chain reactionreverse transcription real-time. This study reveals the pathogen HCV (hepatitis C virus, HCV) in real time. This method consists in the detection of genetic material of the virus - RNA - viral load in blood (total). Detection of HCV RNA in a patient is not talking only about the present, but also the reproduction of the virus in the body. PCR reveals this is ten days after infection.
The quantitative interpretation of the results of HCV RNA and averages:
- 750 copies / ml - no virus in the blood or in a minimum amount;
- . Less than 2 million copies / ml = 800 thousand IU / ml - define low viremia, the prognosis of the disease course best, therapy should be successful;
- . More than 2 million copies / ml = 800 thousand IU / ml - high viremia.
Reliability of assay results can be broken:
- contaminated biomaterial
- chemical and protein inhibitors of substance in the samples;
- Heparin blood.
Deal with the result of the quantitative HCV RNA analysis will help to infectious diseases and a specialist hepatologist.