Decoding PCR Analyzes

Content


PCR analysis and its decoding

PCR (full name — The polymerase chain reaction) is used to identify the pathogens of various infections in the DNA material:

Decoding PCR Analyzes

  • HIV;
  • viral hepatitis of all types;
  • infectious mononucleosis;
  • cytomegalovirus infection;
  • herpes;
  • urogenital infections — ureaplasmosis, chlamydia, garardnellosis, mycoplasmosis, trichomonosis;
  • tuberculosis;
  • Papillomavirus;
  • candidiasis;
  • Liseriosis and other.

Direction for analysis can be given almost any specialist:

  • infectious,
  • dermatovenerologist,
  • gynecologist,
  • urologist,
  • phthisiare,
  • Gastrolologist,
  • Oncologist and others.

Any biological fluid or environment of the body can become a material for PCR:

  • Cal,
  • urine,
  • blood,
  • Allocations taken from genitals and urinary tract,
  • sputum,
  • Spinal fluid,
  • Scuffers of the mucous membranes of all available organs and others.

The essence of the method

The material obtained is placed in amplifier — Special reactor, where, under the influence of certain enzymes, a chain reaction of doubling DNA microbial pathogen. As a result, it becomes quite a lot of it, and this amount gives back to decoding by comparing the result obtained with databases.

Decoding results

PCR analysis results can only be two: positive or negative.
The case is considered to be positive when the DNA of the microorganism is found in the material under study, which caused the disease. This is a sign of either acute infection, or a microbe carrier.
PCR analysis practically does not give erroneous results, it is distinguished by incredible accuracy and sensitivity.

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