HIV transcript analysis

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Deciphering HIV tests

HIV transcript analysis

Human immunodeficiency virus (HIV) can be determined by taking the patient's blood analysis.

The following methods of detecting HIV infection.

Linked immunosorbent assay (ELISA). Apply after three months from the beginning of the infection, otherwise the result will be false. Generation of antibodies with different people occurs at different times: someone - 3 months, and someone - more. For greater effectiveness of ELISA is repeated after 3 months.
Results:
  • false-negative - too early specimen collection, follow-up study will exclude false results;
false positive - is possible in the following diseases:
* Blood diseases
* Recent vaccination,
* Rheumatoid arthritis and others.
To confirm this result shall clarifying analysis by immunoblotting.
  • Immunoblotting - HIV testing as a refinement of the ELISA detects antibodies and separated from them by viruses during electrophoresis.
    Results:
    • negative - no HIV;
    • positive - have HIV.
    • Polymerase chain reaction (PCR) - one of theaccurate blood test for HIV that detects viral genetic material itself. The method allows to detect the virus in three weeks after infection, and does not give false results, being more informative.
    • PCR in real time. The method makes it possible to literally count the viruses in the unit volume of blood. Such precision - this is an opportunity to build a more effective treatment.
      The presence of HIV in the body and tell CBC:
      • high erythrocyte sedimentation rate;
      • anemia;
      • low white blood cells and platelets.
      • Indications for delivery of HIV testing:
        • unsafe sex;
        • sudden weight loss;
        • planning to become pregnant;
        • Preparation for the operation;
        • checkup in certain professions.
        Terms of delivery of blood for HIV:
        • Blood taken from a vein (5 mL);
        • procedure is carried out on an empty stomach in the morning.

        Assign Analysis gynecologist, surgeon, infectious disease specialist, internist. The patient may optionally itself can donate blood for HIV, even anonymously.

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