ELISA and PCR with TORCH-infection
TORCH-infections - microorganisms that cause serious diseases in humans.
- herpes simplex virus;
- . Other infections: hepatitis (B, C, E, D), HIV, chlamydia and syphilis.
Methods of diagnosis of TORCH-infections:
- immunosorbent assay (ELISA);
- polymerase chain reaction (PCR).
ELISA principle - determination of antibodies producedbody in response to infection. The analysis is recorded in advance the specific reaction known pathogen antigen with antibodies in blood samples. Knowing the amount of antigen can be determined antibody titer in the patient's blood. The active infection in the human body, the higher the titer.
Explanation of ELISA tests for TORCH
Titles have numerical value. Depending on the test system manufacturer may take the form of the volume ratio of the titer (e.g., the rate of up to 1: 5) or measured in IU / ml (international units per 1 ml.). Sometimes IU / mL do not written. Normal values are specified in the analysis.
PCR principle - determination in the biologicalmaterial specific pathogen genome. If present in the samples, the conditions for the multiplication of specific nucleic acid sites of infection. Copies can be measured.
Transcription PCR analyzes on TORCH
Decryption can be qualitative (type of responsepositive-negative) and quantitative (number of copies of DNA of the pathogen per unit volume of biological material from a patient in IU / ml). Quantitative PCR analysis to determine the activity of infection, microbial or viral load, and has a numeric value in the event of a positive response.